Many of these women did not arrive in the New World as passive followers. Some, like Isabel Rodríguez, took active roles in medical care during military campaigns, while others, such as María de Estrada, are documented as having fought alongside men in battle. These early female figures embodied a form of frontier bravery that defied European expectations of womanhood. They embraced new identities as settlers, healers, intermediaries, and leaders, asserting influence in the sociopolitical chaos of conquest.
In addition to participating directly in conquest activities, women played crucial roles in the establishment of colonial society. As wives, daughters, and mothers, they helped transplant Spanish customs, language, and religion to foreign soil. Women maintained domestic order in makeshift settlements, raised children amidst uncertainty, and facilitated the early roots of community development. Their presence also helped secure Spanish claims to territory, as familial structures signaled stability and permanence in colonized regions.