Chromosome Structural Analysis: A Practical Approach
maj 1999 · Practical Approach Series Bok 200 · OUP Oxford
E-bok
234
Sidor
family_home
Kvalificerad
info
reportBetyg och recensioner verifieras inte Läs mer
Om den här e-boken
The DNA of eukaryotes is packaged into chromosomes - each chromosome consisting of a very long molecule of DNA and various proteins (e.g. histones), and the number of chromosomes being characteristic for the species concerned. Chromosome analysis can provide a great deal of information for many aspects of cellular genetics such as DNA replication, protein:DNA interactions and genetic manipulation. The book is structured in a methodical fashion - the introductory chapters are centred around analysis of chromatin with chapters on the mapping of protein:DNA interactions in vivo using ligation-mediated PCR and the mapping of chromatin-associated proteins by formaldehyde cross-linking. The next chapters concentrate on the study of whole chromosome structure, including: fission yeast chromosome analysis using FISH and CHIP, isolation of vertebrate metaphase chromosomes and their analysis by FISH, the study of vertebrate chromosome progression through mitosis, and the analysis of mammalian interphase chromosomes by immunofluorescence and FISH. There then follow chapters on FISH in whole-mount tissues and the analysis of the sub-structure of mammalian nuclei in vitro. The final two chapters deal with the experimental manipulation of chromosome structure, including: chromosome assembly in vitro using Xenopus egg extracts and chromosome fragmentation in vertebrate cell lines. This comprehensive and informative laboratory manual includes a diverse range of experimental models for the analysis of chromosomes - such as vertebrates, Drosophila, yeast and Xenopus. Fully illustrated, it focuses on modern techniques and approaches to the study of chromosome structure and will be invaluable to researchers and academic staff in genetics, biomedical science and molecular biology.
Om författaren
Dr Wendy A. Bickmore, Cell Genetics Section, MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU. Tel: 0131 332 2471. Fax: 0131 343 2620. Email: [email protected]
Betygsätt e-boken
Berätta vad du tycker.
Läsinformation
Smartphones och surfplattor
Installera appen Google Play Böcker för Android och iPad/iPhone. Appen synkroniseras automatiskt med ditt konto så att du kan läsa online eller offline var du än befinner dig.
Laptops och stationära datorer
Du kan lyssna på ljudböcker som du har köpt på Google Play via webbläsaren på datorn.
Läsplattor och andra enheter
Om du vill läsa boken på enheter med e-bläck, till exempel Kobo-läsplattor, måste du ladda ned en fil och överföra den till enheten. Följ anvisningarna i hjälpcentret om du vill överföra filerna till en kompatibel läsplatta.
